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Fig. 5 | Biomaterials Research

Fig. 5

From: Psoralen synergies with zinc implants to promote bone repair by regulating ZIP4 in rats with bone defect

Fig. 5

The evaluation of osteogenic activity in Zn + CS/pPRL implants in vitro. (A) MC3T3-E1 cells were cultured with 25% extract of Zn implants or Zn + CS/pPRL implants for 24 h, and the cytoskeleton was stained with FITC labeled phalloidin. (B) the expression of ZIP4 mRNA in MC3T3-E1 cells treated with 25% extract of Zn implants or Zn + CS/pPRL implants for 48 h. (C) Representative image of FluoZin-3-stained MC3T3-E1 cells and fluorescence quantification analysis of intracellular Zn2+ concentrations. (D–E) MC3T3-E1 cells were cultured with 25% extract of Zn implants loaded with CS or/and PRL for 14 d. The cell activity and mineralization of MC3T3-E1 cells were analyzed using alizarin red S staining and ALP staining. Bars represent mean ± S.D. *p < 0.05; **p < 0.01

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Biomaterials Research

ISSN: 2055-7124

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