Fig. 2
PRL regulates the expression of zinc ion transporter ZIP4. (A) Molecular docking of PRL with the protein ZIP4. (B) A cell counting kit-8 (CCK-8) assay was used to detect cell viability of MC3T3-E1 treated with different concentrations of PRL (1-200 µM). (C) The expression of Zip4 mRNA in MC3T3-E1 cells treated with PRL (1, 2, 5 µM). (D) The expression of Zip4 mRNA in MC3T3-E1 cells treated with Zn2+(180 µM) for 24 h, then treated with PRL (1, 2, 5 µM). (E) The level of ZIP4 protein in MC3T3-E1 cells was determined by ELISA. (F) Representative image of FluoZin-3-stained MC3T3-E1 cells, and fluorescence quantification analysis of intracellular Zn2+ concentrations. Bars represent mean ± S.D. *p < 0.05; **p < 0.01