Fig. 7

The in vivo antitumor effects of DOX/HA-b-PCDA NPs on 4T1 breast cancer model. A The relative tumor volume growth curves from each group. B The tumor weight of each group at the end of the treatment. C The mice body weight growth curves from each group. D Histological analysis of tumor tissue by H&E staining, scale bar = 500 μm. E Immunofluorescence staining of apoptosis cells in the tumor tissue at the end of treatment by TUNEL assay, Blue, cell nucleus, Green, apoptosis cell, scale bar = 100 μm. F quantified with Fiji (is just ImageJ) software of apoptosis cells in the tumor tissue at the end of treatment by TUNEL assay in (E). G Immunofluorescent staining of ALDH1^high cells in the tumor tissue at the end of the treatment, Blue, cell nucleus; Green, ALDH1, scale bar = 500 μm. H Fluorescence intensity of ALDH1.^high cells in the tumor tissues (G) quantified by Fiji (is just ImageJ) software. I Western blotting analysis of the ALDH1 expression. J Quantification by densitometry analysis of (H). “*”, p < 0.05; “**”; p < 0.01; “***”, p < 0.001