Fig. 7
From: Post-insertion technique to introduce targeting moieties in milk exosomes for targeted drug delivery
In vivo systemic toxicity of Dox@mExo-FA in HCT116 tumor-bearing mouse. (A-B) Body weight changes in mice during multiple administrations of PBS, mExo-FA, free Dox, Dox@mExo, and Dox@mExo-FA for 16 days (n = 5–8 for each group; black circle: PBS; blue circle: mExo-FA; white circle: free Dox; green circle: Dox@mExo; red circle: Dox@mExo-FA). (C) Spleen size and weight after five consecutive injections of PBS, mExo-FA, free Dox, Dox@mExo, and Dox@mExo-FA. (D) Blood analyses of mice treated with PBS, mExo-FA, free Dox, Dox@mExo, and Dox@mExo-FA for 16 days. (E) Representative H&E images of heart tissue from each group (black dashed line: tissue surface of heart; red arrows: damaged tissue structures; right ventricle (RV)). Scale bar: 100 μm. One-way ANOVA with Tukey’s post-hoc multiple comparisons was performed for statistical analysis: n.s: not significant, **P < 0.01, ***P < 0.001, ****P < 0.0001,