Fig. 5
From: Post-insertion technique to introduce targeting moieties in milk exosomes for targeted drug delivery
In vivo biodistribution of mExo-FA in HCT116 tumor-bearing mouse. (A) Representative fluorescence whole-body images of tumor-bearing mice at various time points (2, 4, 6, 8, 12, and 24 h after systemic 200 µg of mExo/mExo-FA injection). Displayed fluorescence signals were restricted to the tumor area demarcated by black dotted circles. Whole-body fluorescence images are presented in Fig. S7. The radiant efficiency in tumor tissue is calculated by (\(\frac{p/sec/c{m}^{2}/sr}{\mu W/c{m}^{2}}\)). (B) Quantified radiant efficiency of mExo/mExo-FA in primary tumor site (black dotted circle) of tumor-bearing mice in (A) at various time points (2, 4, 6, 8, 12, and 24 h after injection). (C) Ex vivo fluorescence image of major organs (liver, lung, heart, spleen, and kidney) and tumor tissue after 24 h of injection. (D) Quantified radiant efficiency of excised tumor tissues displayed in a red dot box in (C). (E) Representative fluorescence images (left) and quantified fluorescence density graph (right) of F675-labeled mExo/mExo-FA in tumor tissue. Scale bar: 100 μm. The statistical significance was calculated using a two-tailed t-test; *P < 0.05, ***P < 0.001