Fig. 4
From: Post-insertion technique to introduce targeting moieties in milk exosomes for targeted drug delivery
Dox loading and mExo-FA-mediated Dox delivery into HCT116 cells. (A) UV-vis spectra absorbance curves demonstrating the loading capacity of Dox (black line: absorbance of 50 µg Dox; orange line: absorbance of Dox loaded into mExo). (B) Time-dependent cumulative Dox release from mExo-FA and mExo at pH 6.8 and pH 7.4. The release profile of Dox was measured at various time points (10 and 30 min, and 1–5, 8, 10, 20, 24, 30, and 48 h). The red arrow indicates the time point (30 h) of arrival of plateau of Dox release at pH 6.8. (Plateau of Dox release at pH 6.8: 64.6%; at pH 7.4: 50.7%, mExo at pH 6.8: 66.3%; at pH 7.4: 49.2%). * and # indicate statistical significance between pH 6.8 and pH 7.4 in each group (Dox@mExo / Dox@mExo-FA), respectively. (C) Confocal images showing cellular uptake of Dox by Dox@mExo-FA in HCT116 cells (based on 1 µg/mL of Dox concentration). Scale bar: 50 μm. (D) Quantified graph demonstrating intracellular fluorescence intensity of Dox from (C). One-way ANOVA with Tukey’s multiple comparison test was conducted for every statistical analysis: *P < 0.05, ** P < 0.01