Fig. 3
From: Post-insertion technique to introduce targeting moieties in milk exosomes for targeted drug delivery
Receptor-mediated cellular uptake of mExo-FA. (A) Representative fluorescence images indicating binding of mExo and mExo-FA on the surfaces of both cells. Scale bar: 50 μm. (B) Representative fluorescence confocal and bright-field images showing cellular uptake of 50 µg/mL mExo/mExo-FA in HCT116 with FR preblocking with anti-FRα (1:500 v/v ratio). Arrowheads indicate intracellular uptake of mExo/mExo-FA in HCT116 cells. White arrows indicate mExo/mExo-FA stacked (not intracellularly uptaken) on cell surface. (C) Quantified graph indicating intracellular uptake level of mExo/mExo-FA. One-way ANOVA followed by Tukey’s multiple comparisons was performed: *P < 0.05, ***P < 0.001