Fig. 2
From: Post-insertion technique to introduce targeting moieties in milk exosomes for targeted drug delivery
Characterization of the surface functionalized mExo with FA. (A) Schematic illustration showing a process of surface engineering and Dox loading. The box with a blue dashed line demonstrates the modified chemical structure of 16:0 PE-FA obtained from Avanti Polar Lipids. (B) Size distribution of mExo species (mExo, mExo-FA, Dox@mExo, and Dox@mExo-FA). (C) TEM images of mExo, mExo-FA, Dox@mExo, and Dox@mExo-FA. Scale bar: 100Â nm. (D) Western blotting analysis of TSG101, CD9, MFG-E8, calnexin, and casein. TSG101 and CD9, representative positive exosome markers; MFG-E8, milk-exosomal proteins; calnexin, negative marker of exosome; casein, representative contaminants from milk