Fig. 5
ROS generation and tumor cell clearance ability in HTI-labelled HCT-116 cells. A CLSM images depicted the increasing amount of ROS stimulated by HTI under 1 min or 5 min white light irradiation. Blue channel for Hoechst33342: λex = 405 nm, λem = 455 ± 20 nm. Green channel for DCFH-DA: λex = 488 nm, λem = 525 ± 20 nm. Red channel for HTI: λex = 570 nm, λem = 610 ± 20 nm. Scale bar, 10 μm. B CLSM images illustrated the tumor cell clearance ability of HTI under white light irradiation. Blue channel for Hoechst33342: λex = 405 nm, λem = 455 ± 20 nm. Green channel for Calcein-AM: λex = 488 nm, λem = 525 ± 20 nm. Red channel for HTI: λex = 570 nm, λem = 610 ± 20 nm. Scale bar, 10 μm. C The plot of cell viability of HCT-116 cells incubated with HTI at different concentrations with or without white light irradiation, respectively