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Fig. 5 | Biomaterials Research

Fig. 5

From: Mild heat treatment in vitro potentiates human adipose stem cells: delayed aging and improved quality for long term culture

Fig. 5

Lasting effects of mild HT on human AD-MSCs during subculturing without additional HT. a Schematic representation of the experimental design. b metabolic activity, c cumulative cell number, and d doubling time of the MSCs in each group during the subculture. The relative gene expressions of e HSPs (HSP27, HSP70, and HSP90) and f stemness markers (SOX2, OCT4, and NANOG) in each group. g Relative MFI of the positive marker-stained MSCs in each group at P + 6. MFI was obtained using flow cytometry. *p < 0.05 compared to the control group, and #p < 0.05 compared to the HT 41ºC group

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