Scheme 1

Schematic of the whole fabrication process in preparing alginate/ECM/CCM (AEC) patch. The decellularized ECM, ECM was suspended homogenously via ultrasonication, then mixed with 5% sodium alginate solution, subsequently followed by gelation of sodium alginate using 500mM CaCl₂ solution. Meanwhile, concentrated conditioned medium (CCM) was prepared by centrifugation of the conditioned medium of hMSC. Finally, AEC patch was produced by rehydration of the dried alginate/ECM patch using the CCM. Our AEC patch was transplanted in the 8 mm full-thickness wound and extensively assessed for wound healing capability