Fig. 5

Histological analysis of the regenerated wound tissues on day 7 and 14. (A) High magnification images of the regenerated dermis stained by H&E and DAPI staining (inset) on day 7. Scale bar is 50 μm. (B) Medium magnification images of H&E-stained tissue samples. The yellow triangles suggest the neovessels in the dermis region. Scale bar is 100 μm. (C) Low magnification images of H&E samples on day 14. Scale bar is 200 μm. (D) High and lower magnification in the regenerated dermis as assessed by Herovici staining. Blue and purple color indicated young and mature collagen fibers, respectively. Scale bar is 100 μm. (E) Quantification of cellular recruitment in the dermis area on day 7. (F) Quantitative evaluation of the average neovessel size on day 7. (G) Measurement of the epidermis thickness and comparison with normal skin epidermis on day 14. (H) Quantitative analysis of mature collagen deposition in the regenerated dermis on day 14. Statistically significant difference (***p < 0.001, ****p < 0.0001)