Fig. 2
A Images of S. boulardii in bioactive material at 0 h, 18 h, and 36 h, with blank hydrogel as the control group. Bar = 50 μm. B Diagrammatic sketch and histogram of OD600 of S. boulardii in bioactive material. After being cultured in YPD medium for 0 h, 18 h, and 36 h, the sample was decomposed by GelMA lysis buffer for 1 h, and the released S. boulardii was quantitated by the absorbance change at 600 nm. C Fluorescence images of CFW-stained S. boulardii in bioactive material. S. boulardii in bioactive material was stained by CFW, and the distribution of S. boulardii at 0 h and 36 h was observed using a fluorescence microscope. Bar = 50 μm. D 3D reconstructed images of S. boulardii bioactive material obtained by multifunctional fluorescence microscope. Bar = 100 μm. E SEM pictures of S. boulardii bioactive material, where a and b represent the S. boulardii in hydrogel pores. *p < 0.05, **p < 0.01, and ***p < 0.001