Fig. 3

In vivo intratumoral accumulation analysis of LNP candidates administered via MSC guidance (a) The IVIS of 4T1 tumor-bearing mice treated with six LNPs (30 mg/mL, 1 μL) or free Flamma 648 dye (0.15 mg/mL, 1 μL) via MSC guidance showed the time-dependent intratumoral accumulation behaviors of LNPs and Flamma 648 dye. b The in vivo intratumoral fluorescent intensity was measured over the administration time, showing the highest accumulation of 10PS. c The ex vivo fluorescence images of excised tumor tissues 9 h after the MSC-guided LNP treatment were obtained and (d) their fluorescent intensities were quantified. e The intratumoral delivery efficiencies of six LNPs and Flamma 648 dye were calculated by the ratios of tumor-remaining LNP or dye concentrations over those released from the MSC