Fig. 6

HDF-driven fibrotic disease-related proteins in the low elastin hydrogel. A Protein expressions of ECM-, FMT-, and CAF-related proteins in early and late stages. Blue and red squares represent downregulated and upregulated proteins in elastin-variable hydrogels compared to 100C in the early stage, respectively. B qRT-PCR analysis of FHL1, THY-1, and FBN1 gene expression levels in the hydrogels at 0 h and 72 h. C Expression of THY-1 protein (green) in HDFs (red) within hydrogels with or without BAPTA-AM (100 µM) treatment at 0 h and 72 h. D Analytical data derived from the images in C. Data are represented as mean ± SD of triplicate analyses. * p < 0.05