Fig. 6
FRESH bioprinting using SGE 2–1-1 bioink loaded with human neonatal fibroblasts. A (i) Drawing of the printing setup with addition of the crosslinking agents to the ink (+ HRP) and the support bath (H2O2). (ii) Printed grid casted into the support bath and (iii) the same grid after washing away the support bath. B Images of different shapes printed with SGE 2–1-1 C-E (i) Images of 3D bioprinted shapes (C (ii)) Live/Dead staining of encapsulated cells into a grid (8 × 8 mm) on day 1 and day 7. D (ii) Live/dead staining on day 7 shows high cell viability (top view) throughout the construct in a z-stack from the middle area of the cylindrically shaped scaffold (E (ii)) Day 7 LIVE/DEAD staining of a heart shaped grid (iii). Alamar Blue staining of the printed grid showed significantly increasing metabolic activity over time (p < 0.05)