Fig. 2

A biochemical assays for hpcECM characterization. Values are compared to native tissue and show the near complete removal of DNA while preserving matrix components such as collagen and glycosaminoglycans (GAGs). B Determination of chemical modification efficiency. Tyramine peptides were covalently bound to gelatin to create gelatin with enriched phenol groups (GTA). (i) Standard curve for photometric quantification of phenol (Abs280 nm) and (ii) determination of tyramine concentration in GTA compared to gelatin. C-F Biomechanical analysis of four SGE composite hydrogel formulations: SGE 2–0-0 (SF 2%), SGE 2–2-0 (SF 2%, GTA 2%), SGE 2–1.5–0.5 (SF 2%, GTA 1.5%, hpcECM 0.5%), SGE 2–1-1 (SF2%, GTA 1%, hpcECM 1%). C Kinetics of SGE hydrogel formation using fluorescence measurement. D Rheological analysis showing gelation time points. E Uniaxial compression tests and (F) Spectral analyses using Fourier Transformation Infrared Spectroscopy (FTIR)