Fig. 6

In vivo evaluation of early response to treatments and anti-tumor mechanism. A 18 F-FDG PET/CT images of mice in each group, upper: cross-sectional images, lower: coronal images, red circles indicate the tumor sites. SUVmean (B) and SUVmax (C) in each group. ATP secretion (D), CRT exposure (E) and the release of HMGB1(F) in tumor tissues detected by ELISA. Pro-inflammatory cytokine including IL-6 (G), IFN-γ (H) and TNF-α (I) analyzed by ELISA. Apoptosis of tumor tissues stained with DAPI and TUNEL(J), scale bar = 50 μm (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). I: Control II: Bif + NIR + GM-CSF. III: PAu-NPs + NIR + GM-CSF. IV: Bif@PAu-NPs + GM-CSF. V: Bif@PAu-NPs + NIR. VI: Bif@PAu-NPs + NIR + GM-CSF.