Fig. 5
In vivo anti-tumor efficacy evaluation. A Fluorescence imaging and whole-body biodistribution of IR820, L820, and C820 NPS at different time points in the RKO tumor-bearing BALB/c nude mice model. B Fluorescence signal images of main excised organs (heart, liver, spleen, lung, kidney, and tumor) in mice treated with IR820, L820, and C820 NPs at 24h post injection. C and D Infrared thermal images and corresponding temperature variation graph of RKO tumor-bearing nude mice subjected to different treatments followed by laser irradiation for various durations (1, 2, 3, 4, and 5 min; Ctrl and IR820: 808 nm; L820 and C820 NPs: 660 nm; P = 1.0 W/cm2. E Schematic illustration of in vivo treatment on tumor-bearing nude mice. F Tumor volume change graph. G Weight change line chart. H Representative image of isolated tumor from nude mice. Scale bar: 1 cm. I Final tumor volumes and the inhibition rate. J Ki67, TUNEL, and H&E staining of the tumors at the end of anti-tumor studies. Scale bar: 150 μm. All data are shown as mean ± SD and are representative of five independent experiments; n=5, one-way ANOVA, ns P＞0.05, *P < 0.05, **P < 0.01, ***P < 0.001