Fig. 4
Enhancing photochemotherapy synergistic sensitivity through improving tumor cell hypoxia. A CLSM images of LoVo/CDDP cells treated accordingly and stained with hypoxia probes in the presence of 1% O2. Scale bar: 30 μm. B Statistical analysis of mean fluorescence intensity in LoVo/CDDP cells treated with hypoxia and different drugs. C Flow cytometry analysis of intracellular hypoxia level in LoVo/CDDP cells after different treatments in a hypoxic environment. D Immunoblot analysis of hypoxia markers in LoVo/CDDP cells treated with PBS or C820 NPs with or without hypoxia induction. E Immunoblot analysis of NOX2 in LoVo/CDDP cells treated with PBS, CDDP, free IR820, L820, and c820 (Ea) or in RKO, LoVo, and LoVo/CDDP cells treated with C820 NPs (Eb). F Immunoblot analysis of SOD1 in RKO, LoVo, and LoVo/CDDP cells treated with or without C820 NPs and laser irradiation. G Effects of NPs, laser, hypoxia and ferroptosis inhibitors on LoVo/CDDP cells viability. H Fluorescence microscopy images showing LoVo/CDDP cell survival after different treatments. Live cells are marked with calcein-AM (green fluorescence), while dead cells are marked with propidium iodide (red fluorescence). Scale bar: 100 μm. I Statistical analysis of the relative fluorescence ratio of dead cells treated as indicated. Data represent means ± SD (n = 3, one-way ANOVA, **P < 0.01, ***P < 0.001)