Fig. 3
C820 NPs trigger ROS-dependent ferroptosis in colorectal cancer cells. A Fluorescence microscopy images and (B and C) flow cytometry quantitative analysis for intracellular ROS generation of LoVo/CDDP cells using DCFH-DA as a probe. Scale bar: 150 μm. (Ctrl and IR820: 808 nm; L820 and C820 NPs: 660 nm; P = 1.0 W/cm2; irradiation time = 60s). D Confocal laser scanning microscopy（CLSM）images and (E and F) flow cytometry quantitative analysis of the C11-BODIPY (581/591) probe detected lipid peroxidation. Scale bar: 20 μm. (λ = 808 nm for IR820 and 660nm for L820 and C820 NPs, P = 1.0 W/cm2; irradiation time = 60s). G Immunoblot analysis of ferroptosis markers (GPX4) in LoVo/CDDP cells treated as indicated. H-J GSH levels were determined after drugs and irradiation treatment. K Representative transmission electron microscopy images of C820 NPs-induced ferroptosis in colorectal cancer cells. Scale bar: 1 μm/500 nm. (λ = 808 nm for IR820 and 660nm for L820 and C820 NPs, P = 1.0 W/cm2; irradiation time = 60s). Data represent means ± SD (n = 3, one-way ANOVA, **P < 0.01, ***P < 0.001)