Fig. 2
The anti-colorectal cancer effect of C820 NPs. A-C Viability of LoVo/CDDP, LoVo, and RKO cells co-cultured with CDDP, IR820, L820, C820 NPs, and C820 NPs/Hyp under laser irradiation. D IC50 of drugs in different groups of LoVo/CDDP, LoVo, and RKO cells with laser irradiation. E Apoptosis analysis in LoVo/CDDP, LoVo cells treated with PBS, CDDP, IR820, L820, C820 NPs, and C820 NPs/Hyp by Annexin V-FITC/PI double staining and quantification of the proportion of apoptotic cells. (Ctrl and IR820: 808 nm; L820 and C820 NPs: 660 nm; P = 1.0 W/cm2; irradiation time = 60 s). F-I Representative images and quantification of colony formation assay of LoVo/CDDP, LoVo, and RKO cells treated with PBS, CDDP, IR820, L820, C820 NPs, and C820 NPs/Hyp. J Representative images of EdU assay of LoVo/CDDP, LoVo, and RKO cells. Scale bar: 100μm. (K-M) The statistical analysis of the rate of EdU incorporation in LoVo/CDDP (K), LoVo (L), and RKO (M) cells). Data represent means ± SD (n = 3, one-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001)