Fig. 6

In vivo evaluation of treatment outcomes in an orthotopic glioblastoma mouse model. a Immunofluorescence results of tumor-bearing areas stained with anti-occludin antibody. b Mean fluorescence intensity (MFI) of occludin-positive cells. Data are expressed as mean ± SEM (n = 5). c Schematic diagram of the treatment plan. d Nissl staining results. White dashed lines represent the glioblastoma area of the whole brain. e Immunofluorescence results of tumor-bearing areas stained with anti-CD34 antibody followed by DAPI-nucleus counterstaining. f Immunofluorescence results of tumor-bearing areas stained with anti-KI67 antibody followed by DAPI-nucleus counterstaining. g Quantification of tumor volume after treatment. Data are expressed as mean ± SEM (n = 13). h MFI of CD34-positive cells. Data are expressed as mean ± SEM (n = 5). i MFI of KI67-positive cells. Data are expressed as mean ± SEM (n = 5). j Representative image of mouse and brain after treatment. The black arrow indicates the glioblastoma-bearing brain. k Assessment of 30-day survival rate. Data are expressed as mean ± SEM (n = 13)