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Fig. 3 | Biomaterials Research

Fig. 3

From: Self-organized insulin-producing β-cells differentiated from human omentum-derived stem cells and their in vivo therapeutic potential

Fig. 3

Analysis of β-cell markers and insulin secretion. A Gene expression of β-cell progenitor markers. B Transcript-level expression of β-cell maturation markers. C Immunofluorescence staining images of DAPI (blue), PDX-1 (red), and insulin (green). D Analysis of glucose-stimulated insulin secretion in low (2.8 mM) and high-glucose (16.7 mM) conditions. Insulin secretion was calculated by dividing the insulin level in the low- and high-glucose solutions by the total cell lysis insulin level. The insulin secretion index was calculated by dividing the insulin level of high-glucose by the insulin level of low-glucose. Data represent the mean ± SD, *p < 0.05; **p < 0.01; ***p < 0.001. “ns” indicates statistically non-significant. Scale bar = 100 μm. RBP, round-bottom plate; MBP-FGF2, maltose-binding protein-basic fibroblast growth factor 2

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