Fig. 4
From: 3D biofabrication of diseased human skin models in vitro
Representative studies on wounded-skin models. a 2D-based in vitro wound assays and wound-fabrication method using mechanical, optical, electrical, and thermal tools. b Workflow of wound-healing analysis using laser ablation [38]. First, cells were cultured on the plate until full confluency. A circular wound was then created using laser ablation with LEAPâ„¢, followed by rinsing to remove cell debris. Wound closure owing to cellular proliferation and migration was observed by LEAPâ„¢ in the bright-field mode daily. The wound area was calculated and quantified using the texture-segmentation algorithm developed by Smith et al. c Wound-healing analysis was conducted using a laser-irradiated skin model to demonstrate the effect of calcium pantothenate on re-epithelialization [42]. Reprinted with permission from Ref. [38, 42]