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Fig. 3 | Biomaterials Research

Fig. 3

From: Restoration of dysregulated intestinal barrier and inflammatory regulation through synergistically ameliorating hypoxia and scavenging reactive oxygen species using ceria nanozymes in ulcerative colitis

Fig. 3

Hypoxia attenuating and ROS scavenging of PEG-CNPs in intestinal epithelial cells. a Western blot analysis of HIF-1α (β-actin, loading control) in HT-29 cells with different treatment strategies. b qRT-PCR analysis of HIF-1α in HT-29 cells with different treatment strategies. c Migration ability of HT-29 cells with PEG-CNPs treatment. d Relative wound area with PEG-CNPs treatment. e Representative ROS staining of HT-29 cells upon different treatments using DCFH-DA probe. f Quantification of fluorescence of DCFH-DA probe (staining ROS) in HT-29 cells upon different treatments. g Viabilities of HT-29 cells treated with PEG-CNPs and H2O2 show that PEG-CNPs protect cells from oxidative stress-induced cell death. Data represent means ± s.d. n = 3. #P < 0.05, ##P < 0.01, ###P < 0.001 vs. control group; *P < 0.05, **P < 0.01, ***P < 0.001 vs. H2O2 group; no significance (n.s.) vs. normoxia group

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Biomaterials Research

ISSN: 2055-7124

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