Fig. 5

Functional characterization of NK92 2D cultures compared with 3D pore-forming hydrogel after 5 days for cell expansion. a Representative flow cytometric data of cell apoptosis and cell death stained by Annexin V and 7-AAD. b Bar graph of cell viability from flow cytometric data. c Cytotoxicity data of NK92 cell using a Calcein AM-based cytotoxicity assay at different ratio of effector (NK92) to target (K562) (E:T). d Cytokine level of TNF-α and IFN-γ by ELISA. e mRNA expression level of NK92 by qRT-PCR. [n = 3, NS: no significant difference, *p < 0.05, **p < 0.01 and ***p < 0.001]