Fig. 5

In vivo SOR@TF-Fe³⁺ NVs induced ferroptosis, inhibited the growth of tumor, prolonged the survival of mouse in HCC model. A Model diagram of animal experiment and SOR@TF-Fe³⁺ NVs were used for therapy (25 mg NVs/kg). B Changes in tumor volume in mice from the beginning of administration (D7) to the end of administration (D15), n = 5. C Comparison of tumor tissues after administration. D Mouse survival curve of another group of mice treated with the same treatment after discontinuation of D16, n = 5. E Body weight changes of mice during administration, n = 3. F H&E, Ki67, and Prussian Blue staining of tumor tissue, scale: 100 μm. G qPCR analysis of the expression level of ferroptosis indicator ACSL4 mRNA in tumor tissue, n = 3. H Western blot analysis of the expression level of ferroptosis indicator COX2 protein in tumor tissues. Data are expressed as mean ± standard error (SEM), ns: no significant, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, one-way analysis of variance, ANOVA