Fig. 2

Construction and characterization of TF NVs, TF-Fe³⁺ NVs and SOR@TF-Fe³⁺ NVs. A, B qPCR results (n = 3, ***P ≤ 0.001, one-way analysis of variance, ANOVA.) and confocal images (Scale bar: 5 μm) showed the 293T-TF-GFP cells were constructed. C Western blot analysis of GFP protein label expression in 293T cells, 293T NVs, 293T-GFP cells, 293T-GFP NVs, 293T-TF-GFP cells, 293T-TF-GFP NVs protein lysate, WCL: whole cell lysate. D The green spots were observed under confocal. Scale bar: 2 μm. E The morphology of the nanovesicles was observed by transmission electron microscopy (TEM), Scale bar: 100 nm. F Dynamic light scattering (DLS) analysis of the size distribution of TF NVs, TF-Fe³⁺ NVs and SOR@TF-Fe³⁺ NVs. G Phase analysis scattering (PALS) was used to analyze the Zeta potential distribution of all type NVs, n = 3