Fig. 5

Effects of MVF, SVF, and MSC transplantation forin vivoneovascularization in ischemic hindlimb. (A) Immunostaining of ILB4 capillaries (green) and α-SMA blood vessels (red) with nuclei staining by DAPI (blue) in ischemic limbs injected with HBSS, MSCs, SVFs, or MVFs at 28 days after surgery. (B) Quantification of ILB4 and α-SMA double positive arteries in the ischemic limbs by immunostaining (C) Quantification of ILB4 positive capillary in the ischemic limbs by immunostaining. (D) Representative images of ILB4-positive capillaries and α-SMA-positive blood vessels in ischemic limbs transplanted with MSCs, SVFs, or MVFs at 28 days after surgery. Nuclei were counter-stained with DAPI (blue color). The white arrows indicate the HNA-positive blood vessels. (E) Quantification of α-SMA- and HNA-double positive arteries in the ischemic limbs by immunostaining. (F) Quantification of ILB4- and HNA-double positive capillaries in the ischemic limbs by immunostaining. Data is presented as mean ± SD (n = 5). Scale bar = 100 μm ^‡p < 0.005 ^#p < 0.01, *p < 0.05