Fig. 1

Establishment of the protocol for MVF isolation from human adipose tissue (A) Schematic representation of the protocol for MVF and SVF isolation from adipose tissues. The SVFs were serially sieved with 500-µm, 200-µm, and 100-µm pore meshes, and the pass-through fractions and the fractions attached on the meshes were collected. (B) The image of MVFs isolated by sieving with meshes. The MVFs were photographed under a microscope (Scale bar = 400 μm). Dashed lines indicate the MVFs (upper panel). The MVF numbers of the pass-through fractions and the fractions attached on the meshes (lower panel). *p < 0.05 by unpaired t-test (n = 3). (C) Representative images of MVFs (upper panel). Scale bar = 100 μm. The length of MVFs was measured under a microscope and showed as mean ± SD (lower panel). (D) Immunofluorescence staining of MVFs for endothelial markers. MVFs were stained with Ulex europaeus lectin-1 and anti-vWF antibody, counter-stained with DAPI, and the immunofluorescence images were overlaid. Scale bar = 50 μm