Table 3 Overview of display technologies
From: Beyond canonical PROTAC: biological targeted protein degradation (bioTPD)
Screening technologies | Display principle | Selection principle | Typical library size |
|---|---|---|---|
Phage display | Protein/peptide library is displayed on the surface of phage particles | Capture/elution | 109–10 |
Cell-surface display | Protein/peptide library is displayed on the surface of a living cell as a fusion to cell surface protein | FACS selection | 107 eukaryotes 108–10 prokaryote |
Ribosome display | Protein/peptide library is displayed on the stalled ribosome/mRNA complex | Capture/elution | 1012–14 |
mRNA/cDNA display | Protein/peptide library is displayed as covalently attached peptides to its encoding mRNA/cDNA | Capture/elution | 1012–14 |