Fig. 7

Analysis of the long-term cytocompatibility of various embedded endometrial cells within each tissue block. Individual tissue blocks embedded with individual endometrial cells were incubated in their specific culture media for 1, 7, 14, 21, and 28 days after cell encapsulation, and then, the assay solution was added to each sample. Cell viabilities within individual tissue blocks were assessed using a fluorescence microscope. At least 70% of embedded cells within the individual tissue blocks remained viable 28 days after encapsulation (green fluorescence indicates living cells, and red fluorescence indicates dead cells) (A). Similarly, individual tissue blocks were incubated in their specific culture media for 1, 7, 14, 21, and 28 days after cell encapsulation, and then each tissue block sample was additionally incubated with CCK-8 solution in serum-free medium for 48 h (B). All experiments were performed in triplicate. Significant differences are presented. *p < 0.05, **p < 0.005, and ***p < 0.001 (two-sample t-test)