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Fig. 3 | Biomaterials Research

Fig. 3

From: Stabilization and improved functionality of three-dimensional perfusable microvascular networks in microfluidic devices under macromolecular crowding

Fig. 3

MMC promoted the formation of basement membrane enveloping microvascular hollow tubes. Day 4 MVNs were immunostained for basement membrane components, laminin and collagen IV, as well as the focal adhesion component, vinculin, and endothelial cell surface marker CD31. (A) Confocal microscopy images. Scale bar = 200 μm. (B,C) Confocal microscopy images including z-stack images displayed as orthogonal view. Locations of cross-sections are indicated by white lines and orthogonal cross-sections are displayed on the sides. Scale bar = 50 μm. (D) Western blot and densitometric band analysis of laminin and collagen IV normalized to their respective GAPDH levels of samples collected on days 4 and day 7. Protein levels are displayed as fold-change as compared to control. *, p < 0.05. n = 4 biological replicates

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Biomaterials Research

ISSN: 2055-7124

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