Fig. 5
Monitoring of rapamycin-induced autophagy by ASMP-AP. A Images of HeLa cells treated with different concentrations of rapamycin, or inhibitor. λex = 405 nm; λem = 560–620 nm. Scale bars, 20 μm. B, C Quantified fluorescence intensity of panel A (n = 3, by ImageJ software). D Western blot analysis of autophagy-related markers of HeLa cells after different treatments. Statistical analyses were performed with One-way Anova. *: p < 0.05, **: p < 0.01, ***: p < 0.001, and ****: p < 0.0001