Fig. 4From: Ferritin nanoparticles for improved self-renewal and differentiation of human neural stem cellsPromoted differentiation capacity of hfNSC neurospheres by ferritin incorporation. a Immunofluorescence staining for Tuj1 (green) and GFAP (red) of the differentiated hfNSCs near (upper row) and apart from (lower row) the attached neurospheres after 4 days under spontaneous differentiation culture conditions. Cell nuclei were counterstained with DAPI. Scale bar = 200 μm. b qPCR analysis for evaluating gene expression of Tuj1, MAP2, Olig2, and GFAP in differentiated hfNSC neurospheres (n = 3, *p < 0.05 versus No ferritin group)Back to article page